Anti-CD82 Antibody [ARC1501] (A308137)

Name: Anti-CD82 Antibody [ARC1501]
Brand: Antibodies.com
SKU: A308137
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1501] antibody to CD82 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CD82 Antibody [ARC1501]

Description

Rabbit monoclonal [ARC1501] antibody to CD82.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 168-267 of human CD82 (P27701).

Sequence

PEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWPVYQEGCMEKVQAWLQENLGIILGVGVGVAIIELLGMVLSICLCRHVHSEDYSKVPKY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1501

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 - 50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

4F9, Antigen detected by monoclonal and, C33, C33 antigen, CD 82, CD82 antigen, CD82 molecule, CD82_HUMAN, GR 15, GR15, IA 4, IA4, Inducible membrane protein, Inducible membrane protein R2, KAI 1, KAI1, Kangai 1, Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and, Kangai1, Leukocyte surface antigen R2, Metastasis suppressor Kangai 1, Metastasis suppressor Kangai-1, Metastasis suppressor Kangai1, Prostate cancer antimetastasis gene KAI1, R2, R2 leukocyte antigen, SAR 2, SAR2, ST 6, ST6, Suppression of tumorigenicity 6, Suppression of tumorigenicity 6 prostate, Suppressor of tumorigenicity 6, Suppressor of tumorigenicity 6 protein, Tetraspanin 27, Tetraspanin-27, Tetraspanin27, Tspan 27, Tspan-27, Tspan27

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CD82 Antibody [ARC1501] (A308137)

Western blot analysis of extracts of various cell lines, using Anti-CD82 Antibody [ARC1501] (A308137) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-CD82 Antibody [ARC1501] (A308137)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CD82 Antibody [ARC1501] (A308137) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-CD82 Antibody [ARC1501] (A308137)

Immunofluorescence analysis of Jurkat cells using Anti-CD82 Antibody [ARC1501] (A308137) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-CD82 Antibody [ARC1501] (A308137)

Immunofluorescence analysis of Raw264 7 cells using Anti-CD82 Antibody [ARC1501] (A308137) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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