Anti-CD83 Antibody [HB15e] (A280573)

Anti-CD83 Antibody [HB15e]

Mouse monoclonal [HB15e] antibody to CD83.

This antibody recognises the human CD83 cell surface antigen, a 40-45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. In immunohistology CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells co-express the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules and Zhou and Tedder 1995).

IHC-P, IP, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 - 1:100, IHC-Fr: 1:500 - 1:1,000, IHC-P: 1:50 - 1:100 Pack Size: 0.1 mg, 0.2 mg 1:50 Pack Size: 20 µg, Use 10µl of the suggested working dilution to label 10⁶cells in 100µl

Human, Cynomolgus monkey, Chimpanzee, Baboon, Rhesus Monkey, Tasmanian Devil, Sheep

COS cells transfected with HB15 cDNA.

Mouse

Monoclonal

HB15e

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD83 antibody, clone HB15e recognizes the human CD83 cell surface antigen, a 40-45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. In immunohistology CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein (Zhou et al. 1992).However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage (Zhou et al. 1995).CD83+ cells co-express the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules (Zhou et al.1995) and Zhou and Tedder 1995).

B cell activation 45kDa cell surface glycoprotein Ig superfamily, B cell activation protein, B-cell activation protein, BL11, BL11 PEN, CD83 antigen, CD83 antigen (activated B lymphocytes, immunoglobulin superfamily), CD83 molecule, CD83_HUMAN, Cell surface glycoprotein, Cell surface protein HB15, HB15, hCD83