This antibody reacts with an extracellular epitope of CD86, a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages, or activated B lymphocytes.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

B-lymphoblastoid cell line ARH 77.

Host

Mouse

Clonality

Monoclonal

Clone ID

BU63

Isotype

IgG1

Conjugate

PerCP

Excitation: 482nm, Emission: 675nm

Purification

This antibody is conjugated with PerCP under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Predicted MW

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

Activation B7-2 antigen, Activation B7-2 antigen 3, B-lymphocyte activation antigen B7-2, B-lymphocyte activation antigen B7-2 2, B7, B7 2, B7-2, B7.2, B70, B72 antigen, BU63, CD28 antigen ligand 2, CD28 antigen ligand 2 2, Cd28l2, CD28LG2, CD86 antigen, CD86 antigen (CD28 antigen ligand 2, B7-2 antigen), CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2, CD86 molecule, CD86_HUMAN, CLS1, CTLA-4 counter-receptor B7.2, CTLA-4 counter-receptor B7.2 2, 3, Early T-cell costimulatory molecule 1, ETC-1, FUN 1, FUN-1, LAB72, Ly-58, Ly58, MB7, MB7-2, Membrane glycoprotein, MGC34413, T-lymphocyte activation antigen CD86, TS/A-2

Isotype Controls