This antibody recognises mouse CD94, a ~70 kDa cell surface glycoprotein expressed primarily by mouse natural killer (NK) cells. The murine CD94 molecule forms heterodimers with NKG2A, C or E subunits to form the CD94/NKG2 receptor.In mice the CD94/NKG2 receptor recognises Qa-1b, a non-classical MHC class 1 molecule. CD94/NKG2 receptors may play an important role in self-tolerance of NK cells during fetal and neonatal life. N.B. Recent reports suggest that DBA/2J mice do not express cell surface CD94/NKG2A receptors.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Reactivity

Mouse

Immunogen

CHO cells transfected with B6 allele of CD94.

Host

Rat

Clonality

Monoclonal

Clone ID

18d3

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

General Notes

Rat anti Mouse CD94 antibody, clone 18d3 recognizes murine CD94, a ~70 kDa cell surface glycoprotein expressed primarily by mouse natural killer (NK) cells. The murine CD94 molecule forms heterodimers with NKG2A, C or E subunits to form the CD94/NKG2 receptor.In mice the CD94/NKG2 receptor recognizes Qa-1b, a non-classical MHC class 1 molecule. CD94/NKG2 receptors may play an important role in self-tolerance of NK cells during fetal and neonatal life. N.B. Recent reports suggest that DBA/2J mice do not express cell surface CD94/NKG2A receptors (Vance et al. 2002).

Synonyms

CD 94, CD94 antigen, Killer cell lectin like receptor subfamily D member 1, Killer cell lectin-like receptor subfamily D member 1, KLRD 1, KLRD1, KLRD1 protein, KLRD1_HUMAN, KP 43, KP43, Natural killer cells antigen CD94, NK cell receptor, OTTHUMP00000238754, OTTHUMP00000238755, OTTHUMP00000238756, OTTHUMP00000238758, OTTHUMP00000239093

Isotype Controls

Suitable Secondaries

See all Anti-Rat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

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