Anti-Cdc34 Antibody (A14775)

Name: Anti-Cdc34 Antibody
Brand: Antibodies.com
SKU: A14775
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Cdc34 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Cdc34 Antibody

Description

Rabbit polyclonal antibody to Cdc34.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-236 of human CDC34 (NP_004350.1).

Sequence

KWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cdc 34, Cell division cycle 34, Cell division cycle 34 homolog, Cell division cycle 34 homolog (S. cerevisiae), E2 CDC34, UB2R1_HUMAN, UBC 3, UBC3, UBCH3, UBE2 R1, UBE2R1, Ubiquitin carrier protein, Ubiquitin conjugating enzyme Cdc34, Ubiquitin conjugating enzyme E2 32 kDa complementing, Ubiquitin protein ligase, Ubiquitin protein ligase R1, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Cdc34 Antibody (A14775)

Western blot analysis of extracts of Mouse testis, using Anti-Cdc34 Antibody (A14775) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Cdc34 Antibody (A14775)

Western blot analysis of extracts of various cell lines, using Anti-Cdc34 Antibody (A14775) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-Cdc34 Antibody (A14775)

Immunofluorescence analysis of BALB-3T3 cells using Anti-Cdc34 Antibody (A14775) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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