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Anti-CDCA5 Antibody [ARC2096] (A306109)

Western Blot - Anti-CDCA5 Antibody [ARC2096] (A306109) - Antibodies.com Immunohistochemistry - Anti-CDCA5 Antibody [ARC2096] (A306109) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2096] antibody to CDCA5 for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-CDCA5 Antibody [ARC2096]
Description
Rabbit monoclonal [ARC2096] antibody to CDCA5.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 153-252 of human CDCA5 (Q96FF9).
Sequence
GSASTSTPGRRSCFGFEGLLGAEDLSGVSPVVCSKLTEVPRVCAKPWAPDMTLPGISPPPEKQKRKKKKMPEILKTELDEWAAAMNAEFEAAEQFDLLVE
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2096
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CDCA5_HUMAN, Cell division cycle associated protein 5, Cell division cycle-associated protein 5, MGC16386, p35, Sororin
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CDCA5 Antibody [ARC2096] (A306109) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CDCA5 Antibody [ARC2096] (A306109)
Western blot analysis of extracts of HepG2 cells, using Anti-CDCA5 Antibody [ARC2096] (A306109) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-CDCA5 Antibody [ARC2096] (A306109) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CDCA5 Antibody [ARC2096] (A306109)
Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-CDCA5 Antibody [ARC2096] (A306109) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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