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Anti-CDK1 (phospho Thr14) Antibody (A16399)

Western Blot - Anti-CDK1 (phospho Thr14) Antibody (A16399) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to CDK1 (phospho Thr14) for WB.

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Highlighted products for Anti-CDK1 Antibody

Immunohistochemistry - Anti-CDK1 Antibody [POH-1] (A86649) - Antibodies.com
A86650
Anti-CDK1 Antibody [POH-1]
Mouse monoclonal [POH-1] antibody to CDK1 for IP, WB, IHC-P and ICC.
Immunohistochemistry - Anti-CDK1 Antibody [POH-1] (A86649) - Antibodies.com
A86650
Anti-CDK1 Antibody [POH-1]
Mouse monoclonal [POH-1] antibody to CDK1 for IP, WB, IHC-P and ICC.
Name
Anti-CDK1 (phospho Thr14) Antibody
Description
Rabbit polyclonal antibody to CDK1 (phospho Thr14).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T14 of human CDK1 (NP_001777.1).
Sequence
EGTYG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Cdc 2, Cdc2, CDC28A, CDK 1, CDK1_HUMAN, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cell Divsion Cycle 2 Protein, Cyclin Dependent Kinase 1, Cyclin-dependent kinase 1, DKFZp686L20222, MGC111195, p34 Cdk1, p34 protein kinase, P34CDC2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CDK1 (phospho Thr14) Antibody (A16399) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CDK1 (phospho Thr14) Antibody (A16399)
Western blot analysis of HeLa, using Anti-CDK1 (phospho Thr14) Antibody (A16399) at 1:500 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
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