Anti-Cdk4 Antibody [ARC51004] (A81170)

Name: Anti-Cdk4 Antibody [ARC51004]
Brand: Antibodies.com
SKU: A81170
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51004] antibody to Cdk4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Cdk4 Antibody [ARC51004]

Description

Rabbit monoclonal [ARC51004] antibody to Cdk4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 204-303 of human CDK4 (NP_000066.1).

Sequence

FAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51004

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cdk 4, CDK4 protein, CDK4_HUMAN, Cell division kinase 4, Cell division protein kinase 4, CMM 3, CMM3, Crk3, Cyclin dependent kinase 4, Cyclin-dependent kinase 4, Melanoma cutaneous malignant 3, MGC14458, p34 cdk4, PSK J3, PSK-J3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Cdk4 Antibody [ARC51004] (A81170)

Western blot analysis of extracts of MCF7 cells, using Anti-Cdk4 Antibody [ARC51004] (A81170) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-Cdk4 Antibody [ARC51004] (A81170)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cdk4 Antibody [ARC51004] (A81170) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Cdk4 Antibody [ARC51004] (A81170)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cdk4 Antibody [ARC51004] (A81170) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cdk4 Antibody [ARC51004] (A81170)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Cdk4 Antibody [ARC51004] (A81170) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.