Figure 1: Western Blot - Anti-CEACAM5 Antibody [AMC0141] (A306438)
Western blot analysis of extracts of MCF7 cells, using Anti-CEACAM5 Antibody [AMC0141] (A306438) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human tonsil (negative control sample) using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human hepatocellular carcinoma using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human urothelial carcinoma using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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