Anti-CEBP beta (phospho Thr235 + Thr188) Antibody (A95647)

Name: Anti-CEBP beta (phospho Thr235 + Thr188) Antibody
Brand: Antibodies.com
SKU: A95647
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to CEBP beta (phospho Thr235 + Thr188) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-CEBP beta (phospho Thr235 + Thr188) Antibody

Description

Rabbit polyclonal antibody to CEBP beta (phospho Thr235 + Thr188).

Specificity

This antibody detects endogenous levels of C/EBP-beta only when phosphorylated at Thr235/188.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:20000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human C/EBP-beta around the phosphorylation site of Thr235/188 (amino acids 201-250).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

36kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AGP/EBP, C EBP beta, C/EBP beta, C/EBP related protein 2, CCAAT Enhancer Binding Protein beta, CCAAT/enhancer-binding protein beta, CEBPB, CEBPB_HUMAN, CRP2, IL 6DBP, IL6DBP, Interleukin 6 dependent binding protein, LAP, Liver activator protein, Liver enriched transcriptional activator, NF IL6, NFIL6, Nuclear factor NF IL6, Nuclear factor NF-IL6, SF B, SFB, Silencer factor B, TCF-5, TCF5, Transcription factor 5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-CEBP beta (phospho Thr235 + Thr188) Antibody (A95647)

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' using Anti-CEBP beta (phospho Thr235 + Thr188) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-CEBP beta (phospho Thr235 + Thr188) Antibody (A95647)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-CEBP beta (phospho Thr235 + Thr188) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunofluorescence - Anti-CEBP beta (phospho Thr235 + Thr188) Antibody (A95647)

Immunofluorescence analysis of HepG2 cells using Anti-CEBP beta (phospho Thr235 + Thr188) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 4: Western Blot - Anti-CEBP beta (phospho Thr235 + Thr188) Antibody (A95647)

Western blot analysis of various cells using Anti-CEBP beta (phospho Thr235 + Thr188) Antibody.

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