Principle of Assay
Cell Plasma Membrane Staining Kits are a set of fluorescence imaging tools for rapid staining of plasma membranes in living and fixed suspended or attached cells depending on the cell type and experimental conditions. This kit uses a proprietary lipophilic carbocyanine dye (Ex/Em = 549/569 nm) that weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. Enables the uniform staining of cell membrane across a wide variety of mammalian cell types, and can be used for both living and fixed suspended or attached cells. Optimized for various fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. Proprietary CPM Orange™ (Ex/Em = 549/569 nm)-resulting in accelerated diffusion within membranes, staining is also maintained after fixation with formaldehyde, enabling further multi-color staining.
