Anti-CHD3 Antibody [ARC1975] (A307446)

Name: Anti-CHD3 Antibody [ARC1975]
Brand: Antibodies.com
SKU: A307446
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1975] antibody to CHD3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-CHD3 Antibody [ARC1975]

Description

Rabbit monoclonal [ARC1975] antibody to CHD3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CHD3 (Q12873).

Sequence

MKAADTVILWARSKNDQLRISFPPGLCWGDRMPDKDDIRLLPSALGVKKRKRGPKKQKENKPGKPRKRKKRDSEEEFGSERDEYREKSESGGSEYGTGPG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1975

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

260 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

226, 2600010P09Rik, AF020312, ATP dependent helicase CHD3, ATP-dependent helicase Chd3, CHD-3, CHD3_HUMAN, Chd7, Chromodomain-helicase-DNA-binding protein 3, hZFH, MGC40857, Mi 2 autoantigen 240 kDa protein, Mi 2a, Mi-2 autoantigen 240 kDa protein, Mi2 ALPHA, Mi2-alpha, Prp7, Prp9 1, ZFH, Zinc finger helicase, zinc-finger helicase (Snf2-like)

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-CHD3 Antibody [ARC1975] (A307446)

Western blot analysis of extracts of various cell lines, using Anti-CHD3 Antibody [ARC1975] (A307446) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-CHD3 Antibody [ARC1975] (A307446)

Western blot analysis of extracts of Mouse brain, using Anti-CHD3 Antibody [ARC1975] (A307446) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 3: Western Blot - Anti-CHD3 Antibody [ARC1975] (A307446)

Western blot analysis of extracts of Rat brain, using Anti-CHD3 Antibody [ARC1975] (A307446) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 4: Immunohistochemistry - Anti-CHD3 Antibody [ARC1975] (A307446)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-CHD3 Antibody [ARC1975] (A307446) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.