Western Blot - Anti-CHEK2 (phospho S33 + S35) Antibody (A11023)
Western blot analysis of extracts of HeLa and 293 cells, using Anti-CHEK2 (phospho S33 + S35) Antibody (AP0545) at 1:1,000 dilution. HeLa cells were treated by UV for 15-30 minutes. 293 cells were treated by UV for 15-30 minutes. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA.
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