Anti-Chk2 Antibody (A90663)

Name: Anti-Chk2 Antibody
Brand: Antibodies.com
SKU: A90663
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Chk2 for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Chk2 Antibody

Description

Rabbit polyclonal antibody to Chk2.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:20-1:50

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Chk2 (NP_009125.1).

Sequence

MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDS 1, Cds1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHEK 2, Chek2, Chk 2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), CHK2_HUMAN, hCds1, HuCds 1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Chk2 Antibody (A90663)

Western blot analysis of extracts of various cell lines, using Anti-Chk2 Antibody (A90663) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunofluorescence - Anti-Chk2 Antibody (A90663)

Immunofluorescence analysis of HeLa cells using Anti-Chk2 Antibody (A90663). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Chk2 Antibody (A90663)

Immunofluorescence analysis of A549 cells using Anti-Chk2 Antibody (A90663).

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Figure 4: Western Blot - Anti-Chk2 Antibody (A90663)

Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 1µg of Anti-Chk2 Antibody (A90663). This Western blot was performed on the immunoprecipitate using Anti-Chk2 Antibody (A90663) at a dilution of 1:1000.

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