This antibody recognises the beta subunit of cholera toxin.The beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin alpha subunit into cells. The holotoxin consists of a pentameric ring of beta subunits whose central pore is occupied by the alpha subunit. The alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The alpha subunit (and cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
Applications
ELISA, Immunodiffusion
Reactivity
Bacterial
Immunogen
Purified choleragenoid.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
5 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti cholera toxin beta antibody recognizes the beta subunit of cholera toxin.The beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin alpha subunit into cells. The holotoxin consists of a pentameric ring of beta subunits whose central pore is occupied by the alpha subunit. The alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The alpha subunit (and cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
