Anti-Claudin 5 Antibody (A8552)

Name: Anti-Claudin 5 Antibody
Brand: Antibodies.com
SKU: A8552
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Claudin 5 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Intercellular Junction Marker

Specifications

Name

Anti-Claudin 5 Antibody

Description

Rabbit polyclonal antibody to Claudin 5.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 119-218 of human CLDN5 (NP_001349995.1).

Sequence

LTGGVLYLFCGLLALVPLCWFANIVVREFYDPSVPVSQKYELGAALYIGWAATALLMVGGCLLCCGAWVCTGRPDLSFPVKYSAPRRPTATGDYDKKNYV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

20 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Androgen withdrawal and apoptosis induced protein RVP1 like, AWAL, BEC 1, BEC1, Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome), Claudin-5, Claudin5, CLD5_HUMAN, CLDN 5, Cldn5, CPETR L1, CPETRL 1, CPETRL1, TMDVCF, TMVCF, Transmembrane protein deleted in VCFS, Transmembrane protein deleted in velocardiofacial syndrome

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Claudin 5 Antibody (A8552)

Western blot analysis of extracts of Mouse lung, using Anti-Claudin 5 Antibody (A8552) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunofluorescence - Anti-Claudin 5 Antibody (A8552)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Claudin 5 Antibody (A8552) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Claudin 5 Antibody (A8552)

Immunofluorescence analysis of U2OS cells using Anti-Claudin 5 Antibody (A8552) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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