5(6)-CFDA, SE is a cell-permeable fluorescent dye used for cell labeling and tracking in cell proliferation and cell migration studies.
Applications
Flow Cytometry, FACS, Fluorescence Microscopy
Principle of Assay
5(6)-CFDA, SE passively diffuses into cells and is hydrolyzed by intracellular esterases to a fluorescent reactive form that covalently labels intracellular proteins. This enables stable, long-term cell labeling.
Sample Type
Live cultured cells
Assay Time
20-40 minutes
Absorption Maxima
494 nm (pH 7)
Emission Maxima
521 nm (pH 7)
CAS Number
150347-59-4
Molecular Formula
C29H19NO11
Molecular Weight
557.47
Product Form
White solid or pale yellow solid.
Solubility
Soluble in DMSO.
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
