Rabbit polyclonal antibody to 53BP1 (Phospho-Ser25)
Specificity
The antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 25.
Applications
IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 25 (E-D-S(p)-Q-P) derived from Human 53BP1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 53BP1 (Phospho-Ser25) antibody #12125. The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) antibody #12125. The picture on the right is treated with the synthesized peptide.
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