Principle of Assay
This kit provides a simple, convenient and rapid 6-Phosphogluconate dehydrogenase (6PGDH) activity detection method, which is suitable for the detection of animal tissues, plant tissues, cells, bacteria, serum, plasma and other samples. The principle is that 6PGDH catalyzes the reaction of 6-phosphogluconic acid and NADP+ to generate NADPH. NADPH has a characteristic absorption peak at 340 nm, but NADP+ does not; 6PGDH activity is calculated by measuring the increase rate of absorbance at 340 nm.