Rabbit polyclonal antibody to ABL1 / 2 (phospho-Tyr393 / 439)
Specificity
The antibody detects endogenous level of ABL1 / 2 only when phosphorylated at tyrosine393 / 439.
Applications
WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 393 / 439 (D-T-Y(p)-T-A) derived from Human ABL1 / 2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
210kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from HL60 cells using ABL1/2 (phospho-Tyr393/439) Antibody #11530 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2 (Phospho-Tyr393/439) Antibody #11530 (left) or the same antibody preincubated with blocking peptide (right).
