Principle of Assay
Alkaline Phosphatase (AP) Chromogen - Green Enhanced Kit is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing alkaline phosphatase. Alkaline Phosphatase (AP) Chromogen - Green Enhanced has been modified to increase stability and staining intensity, producing a strong green color that is insoluble in alcohol and xylene substitutes (both aliphatic hydrocarbon and citrus based); therefore sections can be dehydrated in alcohol, cleared in xylene substitute, and permanently mounted. However, we recommend air drying slides and then permanently mounting. This chromogen substrate system may be used for both automation and manual use.
