Rabbit polyclonal antibody to AMPK β1 (Phospho-Ser181)
Specificity
The antibody detects endogenous level of AMPK β1 only when phosphorylated at Serine 181.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 181 (L-S-S(p)-S-P) derived from Human AMPK β1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Rat kidney using AMPK β1 (Phospho-Ser181) Antibody #12071.The lane on the right is treated with the antigen-specific peptide.
