Rabbit polyclonal antibody to b-Catenin (Phospho-Ser37)
Specificity
The antibody detects endogenous level of b-Catenin only when phosphorylated at serine 37.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 37 (I-H-S(p)-G-A) derived from Human b-Catenin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from HT29 cells using b-Catenin (Phospho-Ser37) Antibody #11219 and the same antibody preincubated with blocking peptide .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin (Phospho-Ser37) Antibody #11219 (left) or the same antibody preincubated with blocking peptide (right).
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