Principle of Assay
Hematoxylin is a histological staining reagent suitable for visualization of nuclei in tissue sections. Blue Hematoxylin, is a blue nuclear counterstain. Bluing Reagent is used following counterstaining. Blue Hematoxylin Kit is intended for use in the histologic demonstration of nuclear staining. This staining technique is used to make the critical distinction between a normal nucleus and an abnormal one. When used as a counterstain in immunohistochemical procedures, Blue Hematoxylin is compatible with most commonly used chromogens including HRP-Yellow, HRP-Red, HRP-Green, HRP-Black, and DAB. Bluing reagent is intended use for bluing hematoxylin. when applied to tissue sections will change the hue of hematoxylin from purple to blue It replaces the strong alkaline rinses or long tap water washes which may cause loss of cells or tissue sections.
