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Anti-C11B2/CYP11B2 Antibody (A11449)

Western Blot - Anti-C11B2/CYP11B2 Antibody (A11449) - Antibodies.com Immunohistochemistry - Anti-C11B2/CYP11B2 Antibody (A11449) - Antibodies.com Immunohistochemistry - Anti-C11B2/CYP11B2 Antibody (A11449) - Antibodies.com Immunofluorescence - Anti-C11B2/CYP11B2 Antibody (A11449) - Antibodies.com
This product is discontinued

Anti-C11B2/CYP11B2 Antibody (A11449) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-C11B2/CYP11B2 Antibody
Description
Rabbit polyclonal antibody to C11B2/CYP11B2.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B2 (NP_000489.3).
Sequence
TVQLMFMPRSLSRWISPKVWKEHFEAWDCIFQYGDNCIQKIYQELAFNRPQHYTGIVAELLLKAELSLEAIKANSMELTAGSVDTTAFPLLMTLFELARNPDVQQILRQESLAAAASISEHPQKATTELPLLRAALKETLRLYPVGLFLERVVSSDLVLQNYHIPAGTLVQVFLYSLGRNAALFPRPERYNPQRWLDIRGSGRNFHHVPFGFGMRQCLGRRLAEAEMLLLLHHVLKHFLVETLTQEDIKMVYSFILRPGTSPLLTFRAIN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-C11B2/CYP11B2 Antibody (A11449) - Antibodies.comEnlarge Image

Western Blot - Anti-C11B2/CYP11B2 Antibody (A11449)

Western blot analysis of extracts of various cell lines, using Anti-C11B2/CYP11B2 Antibody (A11449) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 20s.
Immunohistochemistry - Anti-C11B2/CYP11B2 Antibody (A11449) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-C11B2/CYP11B2 Antibody (A11449)

Immunohistochemistry analysis of paraffin-embedded rat adrenal gland using Anti-C11B2/CYP11B2 Antibody (A11449) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-C11B2/CYP11B2 Antibody (A11449) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-C11B2/CYP11B2 Antibody (A11449)

Immunohistochemistry analysis of paraffin-embedded mouse adrenal gland using Anti-C11B2/CYP11B2 Antibody (A11449) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-C11B2/CYP11B2 Antibody (A11449) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-C11B2/CYP11B2 Antibody (A11449)

Immunofluorescence analysis of HeLa cells using Anti-C11B2/CYP11B2 Antibody (A11449) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).