Rabbit polyclonal antibody to CaMK2α / β / δ (Phospho-Thr305)
Specificity
The antibody detects endogenous levels of CaMKII only when phosphorylated at threonine 305.
Applications
WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 305 (I-L-T(p)-T-M) derived from Human CaMK2α / β / δ.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from 3T3 cells (Lane 2), using CaMK2α/β/δ (Phospho-Thr305) Antibody #11644. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using CaMKII (Phospho-Thr305) antibody #11644 (left)or the same antibody preincubated with blocking peptide (right).
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