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Anti-Caspase 9 (phospho Thr125) Antibody (A93474)

ELISA - Anti-Caspase 9 (phospho Thr125) Antibody (P12-1085) - Antibodies.com Western Blot - Anti-Caspase 9 (phospho Thr125) Antibody (P12-1085) - Antibodies.com
This product is discontinued

Anti-Caspase 9 (phospho Thr125) Antibody (A93474) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-Caspase 9 (phospho Thr125) Antibody
Description
Rabbit polyclonal antibody to Caspase 9 (phospho Thr125).
Specificity
This antibody detects endogenous levels of CASP9 only when phosphorylated at Thr125.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide derived from human Caspase 9 around the phosphorylation site of Thr125.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
46kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

ELISA - Anti-Caspase 9 (phospho Thr125) Antibody (P12-1085) - Antibodies.comEnlarge Image

Anti-Caspase 9 (phospho Thr125) Antibody (A93474)

ELISA for immunogen phosphopeptide (left) and non-phosphopeptide (right) using Anti-Caspase 9 (phospho Thr125) Antibody.
Western Blot - Anti-Caspase 9 (phospho Thr125) Antibody (P12-1085) - Antibodies.comEnlarge Image

Western Blot - Anti-Caspase 9 (phospho Thr125) Antibody (A93474)

Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 5' + calyculin A 50ng/ml 5' using Anti-Caspase 9 (phospho Thr125) Antibody.