This Catalase Activity Assay Kit is a quantitative assay kit for measuring Catalase activity in serum, plasma, animal and plant tissues and cells.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Catalase converts methanol and hydrogen peroxide to formaldehyde under assay conditions. The formaldehyde reacts with chromogenic reagents to generate a signal measured at 540 nm.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, plant tissues, and cells
Assay Time
30-60 minutes
Components
Assay Buffer, Sample Diluent, Formaldehyde Standard (4.25 M), Catalase (Control), Potassium Hydroxide, Hydrogen Peroxide, Chromogen, Potassium Periodate
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Catalase Activity Assay Kit (A319666)
Representative formaldehyde standard curve for Catalase Activity Assay Kit (A319666). The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (µM).
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