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Anti-CD35 Antibody (A8516)

Western Blot - Anti-CD35 Antibody (A8516) - Antibodies.com Immunofluorescence - Anti-CD35 Antibody (A8516) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD35 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-CD35 Antibody
Description
Rabbit polyclonal antibody to CD35.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 420-550 of human CD35/CD35/CR1 (NP_000642.3).
Sequence
FCPSPPVIPNGRHTGKPLEVFPFGKTVNYTCDPHPDRGTSFDLIGESTIRCTSDPQGNGVWSSPAPRCGILGHCQAPDHFLFAKLKTQTNASDFPIGTSLKYECRPEYYGRPFSITCLDNLVWSSPKDVCK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
270 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
C3 binding protein, C3b/C4b receptor, C3BR, C4BR, CD 35, CD35 antigen, complement component (3b/4b) receptor 1 (Knops blood group), complement component (3b/4b) receptor 1 including Knops blood group system, Complement component receptor 1, Complement receptor 1, Complement receptor type 1, CR 1, CR1, CR1_HUMAN, KN, Knops blood group antigen
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD35 Antibody (A8516) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD35 Antibody (A8516)
Western blot analysis of extracts of U-937 cells, using Anti-CD35 Antibody (A8516) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence - Anti-CD35 Antibody (A8516) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-CD35 Antibody (A8516)
Immunofluorescence analysis of THP-1 cells using Anti-CD35 Antibody (A8516) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-CD35 Antibody (A8516)? Please let us know so that we can list the citation on this page.

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