Rabbit polyclonal antibody to cdc25C (Phospho-Ser216)
Specificity
The antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.
Applications
WB, IHC, ICC/IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from Human cdc25C.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Phospho-Ser216) Antibody #11118 (left) or the same antibody preincubated with blocking peptide (right).
