This kit enables analysis of cell cycle distribution by DNA staining, allowing identification of different cell cycle phases by flow cytometry.
Applications
Flow Cytometry, FACS, Fluorescence Microscopy
Assay Type
Quantitative
Principle of Assay
This kit measures cellular DNA content using a fluorescent nuclear dye whose signal is proportional to nucleic acid content. Differences in fluorescence intensity allow determination of the proportion of cells in G1, S, and G2/M phases.
Platform
Flow Cytometer
Sample Type
Cultured cells
Assay Time
1-2 hours
Components
Nuclear Dye (50X), Assay Buffer (10X), RNase A (100X)
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
General Notes
The optimal Nuclear Dye concentration and incubation time depend on the specific application. Staining conditions may therefore need to be optimized according to the cell type used.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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