Rabbit polyclonal antibody to CHEK1 (Phospho-Ser317)
Specificity
The antibody detects endogenous level of CHEK1 only when phosphorylated at serine 317.
Applications
WB, ICC/IF
Reactivity
Human
Immunogen
A phospho specific peptide corresponding to residues surrounding S317 of human CHEK1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunofluorescence analysis of U2OS cell using Phospho-CHEK1-S317 antibody. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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