Rabbit polyclonal antibody to cofilin1 (phospho-Tyr139)
Specificity
The antibody detects endogenous level of cofilin1 only when phosphorylated at tyrosine 139.
Applications
IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 139 (N-C-Y(p)-E-E) derived from Human cofilin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1 (Phospho-Tyr139) Antibody #11506 (left) or the same antibody preincubated with blocking peptide (right).
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