This Creatine Kinase Activity Assay Kit is a quantitative assay kit for measuring Creatine Kinase activity in animal tissues, cells, and serum.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Creatine kinase generates ATP from creatine phosphate and ADP. Coupled reactions convert ATP into NADPH, and the rate of increase in absorbance at 340 nm is used to calculate enzyme activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, cells, and serum
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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