Cyanine 5.5 is a fluorophore with the emission in the far red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine 5.5. This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in oligonucleotide synthesis. The structure of a phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.
Absorption Maxima
694 nm
Extinction Coefficient
198000 M-1cm-1
Emission Maxima
710 nm
Fluorescence Quantum Yield
0.2
CF260
0.07
CF280
0.03
Purity
85% (by 1H and 31P NMR, and HPLC-MS).
Molecular Formula
C55H71N5ClO3P
Molecular Weight
916.61 kDa
Product Form
Dark colored solid.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
