Principle of Assay
Substrate/chromogen in conjunction with peroxidase-based immunostaining systems. DAB Substrate (Stable) Kit offers several noteworthy improvements and benefits as compared with traditional working DAB solutions. DAB Substrate (Stable) Kit is much more sensitive, providing the cost-effective option of diluting the primary antibody. Being stable for 5 days (as opposed to 6 hours for traditional DAB working solutions), DAB Substrate (Stable) Kit allows the user the convenience of making one working solution for the entire work week. Hazardous waste generation from spent DAB solution is also significantly reduced. DAB Substrate (Stable) Kit is ideal for high volume labs and automated stainers. Peroxidase from the antibody detection system reacts with hydrogen peroxide substrate to degrade it, which then reacts with DAB, precipitating it at positive sites yielding a dark brown color.
