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Anti-DNA Ligase IV / LIG4 Antibody (A92790)

Western Blot - Anti-DNA Ligase IV / LIG4 Antibody (A92790) - Antibodies.com
This product is discontinued

Anti-DNA Ligase IV / LIG4 Antibody (A92790) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-DNA Ligase IV / LIG4 Antibody
Description
Rabbit polyclonal antibody to DNA Ligase IV / LIG4.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:10-1:100
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human DNA Ligase IV (NP_002303.2).
Sequence
SKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
100 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-DNA Ligase IV / LIG4 Antibody (A92790) - Antibodies.comEnlarge Image

Western Blot - Anti-DNA Ligase IV / LIG4 Antibody (A92790)

Western blot analysis of extracts from normal (control) and DNA Ligase IV knockout (KO) HeLa cells, using Anti-DNA Ligase IV / LIG4 Antibody (A92790) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.