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EdU Cell Proliferation Image Kit (Green Fluorescence) (A319642)

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.
Name
EdU Cell Proliferation Image Kit (Green Fluorescence)
Description
This kit detects proliferating cells by labeling newly synthesized DNA with EdU and visualizing them using green fluorescence imaging.
Applications
Fluorescence Microscopy, Confocal Microscopy, High Content Imaging
Principle of Assay
EdU is incorporated into newly synthesized DNA during active DNA replication. Detection is achieved through a copper-catalyzed click reaction between the EdU alkyne group and a fluorescent azide, enabling visualization of proliferating cells without DNA denaturation.
Sample Type
Proliferating cultured cells
Assay Time
1.5-3 hours
Components
EdU (10mM), BSA Wash Solution (5X), Fluor 488 azide, Reaction Buffer (10X), Copper Reagent, Reducing Agent, Hoechst 33342 (1000X), Hydroxyurea
Absorption Maxima
501 nm
Emission Maxima
525 nm
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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