Rabbit polyclonal antibody to eIF4E (Phospho-Ser209)
Specificity
The antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.
Applications
WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
25kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pre-treated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody #11233.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209) Antibody #11233 (left) or the same antibody preincubated with blocking peptide (right).
![Immunofluorescence - Anti-EIF4E Antibody [PCRP-EIF4E-1D3] (A248416) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248416_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-EIF4E Antibody [PCRP-EIF4E-1D3] - BSA and Azide free (A251598) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251598_1.jpg?profile=product_alternative)
