The antibody detects endogenous levels of EPHA2 / 3 / 4 only when phosphorylated at tyrosine 588 / 596
Applications
WB, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 588 / 596 (K-T-Y(p)-V-R) / (R-T-Y(p)-V-D) derived from Human EPHA2 / 3 / 4.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody #11690.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody #11690.
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