Ethidium Homodimer-1 (EthD-1) (A319644)

Ethidium Homodimer-1 (EthD-1)

Ethidium Homodimer, is a high affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it can not cross cell membranes to stain living cells. However, it is very useful to detect nucleic acids in solution, or cells with disintegrated cell membranes.

528 nm (with DNA), 493 nm (in water without DNA)

617 nm (with DNA)

C₄₆H₅₀Cl₄N₈

857

Red solid.

Soluble in DMSO or MeOH.

Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!

This product is for research use only. It is not intended for diagnostic or therapeutic use.