EthD-1 is a nucleic acid stain that penetrates damaged cell membranes and produces red fluorescence in dead cells, making it useful for cell viability assays.
Applications
Fluorescence Microscopy, Flow Cytometry
Principle of Assay
Ethidium Homodimer-1 is a high-affinity nucleic acid dye that cannot cross intact plasma membranes, making it useful for staining dead or membrane-compromised cells. Fluorescence increases strongly upon binding DNA or RNA.
Sample Type
Dead or membrane-compromised cells
Assay Time
10-20 minutes
Absorption Maxima
528 nm (with DNA), 493 nm (in water without DNA)
Emission Maxima
617 nm (with DNA)
CAS Number
61926-22-5
Molecular Formula
C46H50Cl4N8
Molecular Weight
857
Product Form
Red solid.
Solubility
Soluble in DMSO or MeOH.
Storage
Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
