Rabbit polyclonal antibody to Ezrin (Phospho-Tyr353)
Specificity
The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
81kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin (Phospho-Tyr353) Antibody #11063 (left) or the same antibody preincubated with blocking peptide (right).
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