Western blot analysis of extracts of various cell lines, using Anti-Filamin A Antibody (A12186) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Immunohistochemistry - Anti-Filamin A Antibody (A12186)
Immunohistochemistry analysis of paraffin-embedded human colon muscle using Anti-Filamin A Antibody (A12186) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Filamin A Antibody (A12186)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Filamin A Antibody (A12186) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Filamin A Antibody (A12186)
Immunohistochemistry analysis of paraffin-embedded human uterine cancer using Anti-Filamin A Antibody (A12186) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Alternative products to Anti-Filamin A Antibody (A12186)
