This gamma Glutamyl Cysteine Ligase Activity Assay Kit is a quantitative assay kit for measuring gamma Glutamyl Cysteine Ligase activity in serum, plasma, animal and plant tissues, cells, bacteria, and fungi.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Gamma-glutamyl cysteine ligase catalyzes synthesis of gamma-glutamylcysteine from glutamate and cysteine in the presence of ATP and Mg2+. Activity is determined from the rate of inorganic phosphate formation.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, plant tissues, cells, bacteria, and fungi
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Standard
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Representative standard curve for gamma Glutamyl Cysteine Ligase Activity Assay Kit (A319760). The x-axis is OD660 and the y-axis is inorganic phosphorus concentration (µmol/mL).
Publishing research using gamma Glutamyl Cysteine Ligase Activity Assay Kit (A319760)? Please let us know so that we can list the citation on this page.
