This Glucose Oxidase Activity Assay Kit is a quantitative assay kit for measuring Glucose Oxidase activity in serum, plasma, animal tissues, cells, bacteria, and other biological fluids such as urine.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Glucose oxidase converts glucose to hydrogen peroxide, which then reacts with a chromogenic reagent under acidic conditions. The colored product is measured at 580 nm and reflects enzyme activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, cells, bacteria, and urine
Assay Time
30-60 minutes
Components
Assay Buffer (20X), Glucose (0.2 M), Hydrogen Peroxide Standard (0.88 M), Chromogen, Glucose Oxidase (Control)
Storage
Shipped at 4°C. Store at -20°C in the dark for up to 12 months from receipt. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Glucose Oxidase Activity Assay Kit (A319680)
Representative standard curve for Glucose Oxidase Activity Assay Kit (A319680). The y-axis is OD580 and the x-axis is hydrogen peroxide concentration (µM).
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