This Glutamic Acid Dehydrogenase Assay Kit is a quantitative assay kit for measuring Glutamate Dehydrogenase activity in serum, plasma, tissues, cells, plants, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Glutamate dehydrogenase catalyzes conversion of alpha-ketoglutarate, ammonium, and NADH to glutamate and NAD+. The decrease in absorbance at 340 nm is used to calculate enzyme activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, tissues, cells, plants, and bacteria
Assay Time
30-60 minutes
Components
Reagent I, Reagent II, Reagent III
Storage
Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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