This Glutathione Peroxidase Activity Assay Kit is a quantitative assay kit for measuring Glutathione Peroxidase activity in serum, plasma, plant tissue/cell lysates, and other biological fluids.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Glutathione peroxidase oxidizes GSH in the presence of peroxide, and the resulting GSSG is recycled by glutathione reductase with concurrent NADPH consumption. Activity is calculated from the decrease in absorbance at 340 nm.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, plant tissues, cell lysates, and biological fluids
Assay Time
30-60 minutes
Components
Assay Buffer, Substrate, GR, Hydrogen Peroxide
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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