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Anti-HHV6AgQ1 Antibody (A340)

Identification of gQ1 in HHV-6A infected cells by western blotting using anti-gQ antibody (119). T-cell line HSB-2 cells were infected with HHV-6A at m.o.i of 0.1 and the cells were harvested at 72 h postinfection for lysate preparation for WB. gQ1 is detected as two glycoproteins with 80 kDa and 74 kDa molecular masses.. Sample lysates: (-) Non-treated. (H) Treated with end-glycosilase H. (F) Treated with peptide N-glycosidase F. Fig.2. Immunoprecipitation of gQ1 from HHV-6A infected cell lysate with anti-gQ1 antibody (119). gQ1 protein was precipitated from the lysate of infected HSB-2 cells by using agarose beads-conjugated anti-gQ1 antibody (119), and processed for western blotting by using anti-gQ1 antibody (119). Lane 1; HSB-2 cells. Lane 2; HSB-2 cells infected with HHV-6A GS strain Immuno-staining of gQ1 in HHV6A infected HSB-2 cells by using anti-gQ1 antibody. The infected cells were harvested 3 days postinfection, fixed in cold acetone and immunostained with FITC-conjugated anti-gQ1 antibody (119). Specific immunofluorescence was observed with a confocal laser scanning 4. Flow cytometry analysis of gQ1 expression on the cell surface in a transient expression system. 293T cells were transfected with gQ1 expression plasmid. The cells were harvested on second day postinfection, fixed with 4% PFA, permeabilized with 0.1% Triton-X100, incubated with anti-gQ1 antibody (119) and then with FITC-conjugated anti-mouse IgG antibody. Histograms show fluorescence intensities measured in arbitrary units on a log scale (x axis) and relative cell numbers on a linear scale (y axis). Black line is control and red line is gQ1 introduced cells.
$590
Product Datasheet

Mouse monoclonal (119) antibody to HHV6AgQ1 for WB, IP, ICC/IF, FC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-HHV6AgQ1 Antibody
Description
Mouse monoclonal (119) antibody to HHV6AgQ1.
Specificity
This antibody reacts with gQ1 of HHV6A and HHV6B, however, this antibody is not recommended for IP of HHV6B due to conformation specificity.
Applications
WB, IP, ICC/IF, FC, ELISA
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:100-1:3,200, FC 1:100
Immunogen
Recombinant gQ1 of HHV6A protein (a.a. 3-422, His tag), expressed in E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
119
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Purity
> 90% (by SDS-PAGE)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Identification of gQ1 in HHV-6A infected cells by western blotting using anti-gQ antibody (119). T-cell line HSB-2 cells were infected with HHV-6A at m.o.i of 0.1 and the cells were harvested at 72 h postinfection for lysate preparation for WB. gQ1 is detected as two glycoproteins with 80 kDa and 74 kDa molecular masses.. Sample lysates: (-) Non-treated. (H) Treated with end-glycosilase H. (F) Treated with peptide N-glycosidase F.Enlarge Image

Anti-HHV6AgQ1 Antibody (A340)

Anti-HHV6AgQ1 Antibody
Fig.2. Immunoprecipitation of gQ1 from HHV-6A infected cell lysate with anti-gQ1 antibody (119). gQ1 protein was precipitated from the lysate of infected HSB-2 cells by using agarose beads-conjugated anti-gQ1 antibody (119), and processed for western blotting by using anti-gQ1 antibody (119). Lane 1; HSB-2 cells. Lane 2; HSB-2 cells infected with HHV-6A GS strainEnlarge Image

Immunoprecipitation - Anti-HHV6AgQ1 Antibody (A340)

Anti-HHV6AgQ1 Antibody
Immuno-staining of gQ1 in HHV6A infected HSB-2 cells by using anti-gQ1 antibody. The infected cells were harvested 3 days postinfection, fixed in cold acetone and immunostained with FITC-conjugated anti-gQ1 antibody (119). Specific immunofluorescence was observed with a confocal laser scanningEnlarge Image

Anti-HHV6AgQ1 Antibody (A340)

Anti-HHV6AgQ1 Antibody
4. Flow cytometry analysis of gQ1 expression on the cell surface in a transient expression system. 293T cells were transfected with gQ1 expression plasmid. The cells were harvested on second day postinfection, fixed with 4% PFA, permeabilized with 0.1% Triton-X100, incubated with anti-gQ1 antibody (119) and then with FITC-conjugated anti-mouse IgG antibody. Histograms show fluorescence intensities measured in arbitrary units on a log scale (x axis) and relative cell numbers on a linear scale (y axis). Black line is control and red line is gQ1 introduced cells.Enlarge Image

Anti-HHV6AgQ1 Antibody (A340)

Anti-HHV6AgQ1 Antibody
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