Principle of Assay
High Contrast DAB is highly sensitive, providing the cost-effective option of further diluting the primary antibody. Being stable for 2 weeks (as opposed to 6 hours for traditional DAB working solutions), High Contrast DAB allows the user the convenience of making one working solution for the entire week’s work. Hazardous waste generation from unused DAB solution is also significantly reduced. High Contrast DAB is ideal for high volume labs and automated stainers. High Contrast DAB stains dark chocolate brown and is easy to distinguish between other chromogens such as our Chromogen - Red and Chromogen - Blue series when multiple chromogens are used on a single slide. 3,3'-Diaminobenzidine (DAB), is a widely used chromogen for immunoperoxidase staining, and is well accepted among pathologists because of its increased sensitivity and decreased background when compared to amino ethylcarbazole (AEC). Specimens stained with DAB can be dehydrated, cleared, and permanently mounted. High Contrast DAB is more sensitive and stable than traditional working DAB solutions. High Contrast DAB offers several noteworthy improvements and benefits as compared to traditional working DAB solutions. Substrate/chromogen in conjunction with peroxidase-based immunostaining systems. In the presence of hydrogen peroxide, DAB is converted to an insoluble brown reaction product and water by the enzyme horseradish peroxidase (HRP).
