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Anti-His-Tag Antibody (A50037)

His-Tag Mouse Monoclonal Antibody from Signalway Antibody (T505) - Antibodies.com
$290
Product Datasheet

Mouse monoclonal antibody to His-Tag for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-9 business days.

  • Specifications

Name
Anti-His-Tag Antibody
Description
Mouse monoclonal antibody to His-Tag
Specificity
His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6His -tagged proteins.
Applications
WB, ICC/IF
Dilutions
WB: 1:3000~1:10000
Reactivity
Species Independent
Immunogen
A synthetic peptide HHHHHH coupled to KLH.
Host
Mouse
Clonality
Monoclonal
Conjugate

Unconjugated

Purification
Affinity-chromatography
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

His-Tag Mouse Monoclonal Antibody from Signalway Antibody (T505) - Antibodies.comEnlarge Image

Anti-His-Tag Antibody (A50037)

IP antibody use：5ug His Mouse IgG1 per ml Lysate, WB 1: 30001) untransfected 293 cell lysate2) transfected 293 cell lysate with His-tag fusion protein3) IP (untransfected 293+anti-His mAb+ Protein G agarose)4) IP (transfected 293+ normal Mouse IgG+Protein G agarose)5) IP (transfected 293+anti-His mAb+ Protein G agarose)6) Recombinant protein (E.coli)
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